Technologies
Other equipment
Single-cell system
Single-cell analysis addresses biological questions that have previously been impossible to answer. The platform is equipped with the following system:
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Chromium X
The high throughput Chromium X system from 10x Genomics allows profiling of tens of thousands of single cells - fixed cells included - at a time thanks to the Gel Bead-in-Emulsion (GEM) technology (gel bead partitions or vesicles in which barcoded cDNA is prepared). The preparation of NGS libraries is then carried out in bulk reaction (see further details in 10x Genomics website).
10x Genomics Chromium X
We are grateful to the Ernst et Lucie Schmidheiny & Carlos et Elsie de Reuter Fondations for their support.
Photo 10x Genomics
2100 Bioanalyzer and 2200 TapeStation
We routinely use the Agilent 2100 Bioanalyzer and 2200 TapeStation for quality control of total, messager or small RNA and of DNA samples before any application. Using electrophoresis technologies (in capillaries for the 2100 instrument), these instruments rapidly inform about RNA integrity and DNA size fragments in a quantitative way, using much less material compared to traditional electrophoresis gel.
QuBit Flex fluorimeter
In order to quantify very low quantities of material (e.g. FACS-sorted cells, ChIP material, ...), the Genomics Platform is equipped with the QuBit Flex fluorimeter from Invitrogen. This instrument is able to quantify with high precision and sensitivity nucleic acids in solutions without prior purification of the samples, using fluorescent specific dyes for RNA or DNA.
Covaris
The Covaris technology is based on the Adaptive Focused Acoustic (AFA) process established as the DNA shearing method, the first step in the preparation of NGS DNA sequencing libraries. It ensures the integrity of nucleic acid samples providing high recovery of double-stranded DNA. This technology allows to precisely and accurately fragment DNA and RNA from 100 bp to 5 kb.
Pipetting robots
- Three Freedom EVO from Tecan.
Tecan Freedom EVO 150
Photo Tecan
